Mass Spec protocol
09 July 2008
A new Mass Spec protocol has been added to the practical advice section. This protocol is applicable to any protein complex that can be isolated to sufficient purity and quantity, its components separated by gel electrophoresis and sequence databases for the source organism are available. Using this protocol, repeated LC-MS/MS analysis of an SDS-PAGE gel band identifies 90% of proteins in all three replicate and 94% in two out of three replicate LCMS/MS experiments.
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