4-steps to Fixing Proteomics
Now you understand the challenges of running proteomics experiments that can be relied upon and reproduced across-labs you may be asking what you can do about it. Hint: if you're still unsure then check out the simple analogies.
By remembering the analogies, considering your quantitative proteomics experiment design and adopting the 4-steps to Fixing Proteomics you'll build in across-lab reproducibility from the start. This means you can generate results with real impact.
The table summarises the 4-steps, where they can fit into your experimental approach and questions you can use to check you've achieved each step. Print out a copy for your lab.
| Step | Experiment Phase | Typical replicate numbers | Have I reached this step? |
1. Check your system Step 1 |
Basic technical functionality | 1 | Are you confident you get the same result every time you run a standard on your system? |
2. Run pilot experiments Step 2 |
Exploratory | >3 | Do you know everything you need to design and run a conclusive experiment? |
3. Confirm your results Step 3 |
Within lab confirmatory | Calculated from pilot | Can you reliably reproduce and confirm the results you got from your pilot using enough replicates? |
4. Reproduce across-lab Step 4 |
Cross lab confirmatory | Calculated from pilot and confirmatory | Are you confident these results are biological in nature, reproducible by the community as a whole and are not influenced by specific protocols within your lab? |
How did you do?
If you answer "YES" to every question then you're FIXING PROTEOMICS! Tell us and you'll get a special place on our Founders page and a FREE Fixing Proteomics t-shirt like these. If, like most people we talk to you cant answer every question confidently then don't worry this site can help you.
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