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Protocols for 2D PAGE proteomics

Running standards and applying standard procedures as part of your experimental approach delivers proteomics experiments that you can rely on. Here you can find out how to prepare a protein sample and separate proteins using standard procedures proven to generate results that are reliable and reproducible across-labs.

How to prepare a standard protein sample
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How to prepare a standard protein sample
This is probably the most critical and variable step in quantitative proteomics analysis experiments. Download a description of how to prepare a reference sample that can be used for any mammalian cell line and almost unmodified for many mammalian tissue samples as well. We don't claim this is the 'best' procedure but it is proven to be very robust across a wide range of samples...
 
How to run a 2D gel reproducibly
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How to run a 2D gel reproducibly
2D-PAGE is a powerful technique for generating reliable results and the only one currently proven to be reproducible across-labs. Download a easy-to-follow method to running high quality 2D-gels. The procedure described here has been proven and we guarantee it will work with most common samples prepared using the sample preparation protocol above...

See also: 2D gel image capture.

Protocols provided by Sjouke Hoving and Hans Voshol, Novartis Institutes for BioMedical Research, Basel, Switzerland