http://www.fixingproteomics.org/forum/recent.aspx Fixing Proteomics - Recent Posts en-us http://blogs.law.harvard.edu/tech/rss Jitbit AspNetForum Fri, 09 Mar 2012 08:43:21 GMT Fri, 09 Mar 2012 08:43:21 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=10 Our company (Bioproximity, LLC) specializing in biological mass spectrometry for protein analysis and proteomic informatics. We offer a wide range of proteomics services customized to the needs of our clients.
On our website (http://www.bioproximity.com/) there are forms for a variety of questions about proteomics and our works.
Welcome!
edited by swensson on 09/03/2012]]> Fri, 09 Mar 2012 08:43:21 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=9 I used coomassie even colloidal coomassie staining for my 2D gels, but unfortunately I have poor results. Therefore, I prefer to use silver staining. Although I carried out a mass compatible silver staining protocol, I cannot get a good MS/MS result. I'm new in mass spec analysis and my problem is there are several mass compatible silver staining protocols and I cannot decide which to prefer. For instance, some protocols use formalin in each step but the others use it only in developing step. Some uses only EDTA in stopping step, the others use acetic acid. Therefore, I need people who have good mass spec analysis using silver stained spots.
Thanks in advance..
Burcu]]> Wed, 21 Sep 2011 21:54:28 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=8
Thank You.

Regards,

Pushpa]]> Mon, 13 Sep 2010 13:49:46 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=8 Mon, 13 Sep 2010 08:38:18 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=8 Tue, 07 Sep 2010 13:59:56 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=8
sjouke]]> Tue, 07 Sep 2010 13:23:54 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=8
1) Sample: Depleted Plasma ( The plasma has been depleted by using Hi Trap Albumin & IgG removal Kit from GE)
2) pH of the IPG strip is pH 3-10 NL
3) Its a precast gradient gel ( 4-15%) (Bio-Rad)
4) Protein concentration:100ug
5) staining method: Coomassie brilliant blue

4) First dimension steps:
The steps were:

Step 1: 150V, Linear, 15minutes.

Step 2: 500V, linear, 30 minutes

Step 3: 100V, linear, 30 minutes

Step 4: 4000V, linear, 1hr 30 minutes

Step 5: 4000V, Rapid, Vhrs:10000

Step 6: Holding steps.



Hope to get the reply asap.

Thanx alot.

Regards,
Pushpa.]]> Thu, 26 Aug 2010 11:18:10 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=7 Continue reading...
edited by FixingProteomics on 07/10/2009]]> Wed, 07 Oct 2009 15:37:31 GMT http://www.fixingproteomics.org/forum/messages.aspx?TopicID=6 Sample preparation - a necessary evil or the key to proteomics?

Over the years, the main focus of proteomics has been on technological aspects such as capacity and dynamic range of 2D gels, masspectrometry resolution and sensitivity. Enormous amounts of money and effort have been spent on this work while the softer side of proteomics; study design, sample preparation and reproducibility have been left out in the cold. The old saying "garbage in, garbage out" holds as true for proteomics as other parts of life, no matter how many millions of Euros you spend on for instance mass spectrometers. In order to fix proteomics and release its full inherent potential these softer aspects must be allowed a place on centre stage."

Continue reading...]]> Thu, 30 Apr 2009 14:31:44 GMT