Fixing Proteomics Campaign

Fixing Proteomics Campaign - help proteomics deliver its promise to aid successful drug discovery research Send to a colleagueNotify me when this site is updated

Show your support

Fixing Proteomics is being actively promoted by a growing number of people and companies. You can send in your pictures and ideas for things we can add to the website by emailing us or give us your feedback and help promote Fixing Proteomics.

Fixing Proteomics at HUPO 2007 Fixing Proteomics at SPS 2008 Fixing Proteomics on Everest Fixing Proteomics at ABRF 2008
Nonlinear Dynamics - active contributors to Fixing Proteomics
Reproducibility Studies
Phase 1 & 2
  Denator - active contributors to Fixing Proteomics
Sample prep editorial & Forum
  Bio-Rad - active contributors to Fixing Proteomics
Reproducibility Study Phase 2
  gelcompany - active contributors to Fixing Proteomics

Add your voice

We like to hear what you think about Fixing Proteomics and how it helps you. Send us a quote to post here or talk with others on our forum or via the Fixing Proteomics Facebook group.

"Over the years, enormous amounts of money and effort have been spent on the technological aspects of proteomics, such as throughput, dynamic range and resolution. In order to further advance the field of proteomics, we must now focus on the softer sides such as study design, standardisation, sample selection and preparation. The Fixing Proteomics Campaign is a very good way to shed light on these matters and we are happy to support this cause" Dr Mats Borén, Head of Development, Denator AB, Sweden

"An excellent web site that supports both undergraduate teaching and research" Dr. Noel Carter, Faculty of Applied Sciences, University of Sunderland, UK

"I would be more than happy to help promote increased awareness of the issues related to conducting successful and reliable proteomics experiments"Massimiliano Gentile, Biomedicum Genomics, Helsinki University, Finland

"I wholeheartedly support the campaign to further proteomics as an extremely important area of research and development in the drive for improvements in our understanding of the complex interactions in the human body. New drug discovery will be underpinned by proteomics and as such this is an essential field which needs to be nurtured, developed and supported wherever possible." Professor John MacIntrye, Dean of the Faculty of Applied Sciences, University of Sunderland, UK

"The Fixing Proteomics Campaign website represents a valuable resource, annotated by leading figures in proteomics from industry and academia, for anyone interested in carrying out reliable and reproducible proteomics experiments. The protocols and practical advice on designing proteomics experiments should prove useful to newcomers and veterans alike." Professor Mike Dunn, SFI Research Professor of Biomedical Proteomics Proteome Research Centre, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin

"One of the most important steps in any proteomics study is the experimental design - get it wrong and huge amounts of resources may wasted. Creating a suitable experimental design depends on many factors and one of these is most definitely how well the experimental procedures are executed and hence reproducibility. This website not only provides useful information on how to improve reproducibility, but also suggestions of what to think about when designing a quantitative proteomics experiment." Dr. Kathryn Lilley, Facility Group Leader of the Cambridge Centre for Proteomics, University of Cambridge and a campaign Founder

"I think that the Fixing Proteomics Campaign is a great way to raise awareness of reproducibility problems associated with conducting large scale analysis of proteins. Other groups such as the ABRF Proteomics Standards Research Group and the HUPO Proteomics Standards Initiative are crucial for the development of robust and standardised proteomic work flows, data analysis and reporting of data. The complexity of proteomics experiments in terms of the large number of types of experiments, analysis methods and many instrumental platforms makes this process of standardisation much more difficult than that it has been for genomics but it is essential to allow progress in the field." Dr. Mark Collins, from the Proteomic Mass Spectrometry Group at The Wellcome Trust Sanger Institute

"I am passionate about proteomics and feel that we do need to work together and apply universal standards in order to change mass opinion so I am wholeheartedly in favour of this web site." Dr. Balwir Matharoo-Ball,John Van Geest Cancer Research Centre, Nottingham Trent University

"A very good campaign. I am happy to support this" Dr. Miriam Dwek, School of Biosciences, University of Westminster

"I heard about the website on the ABRF discussion group and fully support your campaign. At our lab we make a point of validating methods properly, using both technical and biological replicates and incorporating QC samples in any quantitative proteomic study. I hope you reach as many people as possible because it is possible to do things properly." Dr. Yishai Levin, Institute of Biotechnology, The Cambridge Centre for Neuropsychiatric Research, Bahn Laboratory

"I have been doing comparative protein expression work (Proteomics now) for over 22 years and I still find it unbelievable that researchers so not run appropriate positive and negative controls. I have spent the last 15 years in industry and have found that nearly 60% of the published protocols on purification of proteins from various sources are completely and totally irreproducible. I am happy to see an organization that would like to make all Proteomic work as solid as the old peer-reviewed publications." Richard Mehigh, Sigma-Aldrich Biotechnology

"As Operations Manager at APAF I see the availability of a commercially produced quality assurance program in which a range of standard materials is assayed across as many sites as possible and the results statistically compared, as being integral to giving credibility to proteomics. This is exactly the kind of proficiency testing that underpins accreditation with the likes of NATA (in Australia) by ensuring result reproducibility. Accreditation assures customers the laboratory meets a given standard which allows them the confidence to invest in proteomic services." Keiran Wicks, Operations Manager, Australian Proteome Analysis Facility – APAF at Macquarie University

"Proteomics has a future - you have just got to be very careful with experimental design and number of replicates. We have got some good insights from proteomics which would have been missed by a DNA array approach. The main thing is that it is not just about techniques and instrumentation but the entire approach which counts." Professor Toni Slabas, School of Biological and Biomedical Sciences, Durham University

"We tend to under-estimate the complexity of biology - proteomics is just a mirror of this complexity. Therefore, don't blame proteomics if you don't find your biomarkers of a complex disease! Modern 2D gel technology has a given position in the future of expression proteomics and biological inter-individual variability should always be build into the experimental design." Dr. Bo Franzén, Assoc. Principal Scientist, Target Biology,  Molecular Pharmacology Local Discovery,  Research Area CNS & Pain Control AstraZeneca R&D, Sweden